RESUMO
Endometrial differentiation of the implantation site (IS) is regulated by hormonal and local influences (egg). To better understand the possible participation of cyclic nucleotides in this process, we measured cAMP and cGMP by RIA in IS of the rat on day 6 of pregnancy. IS showed an increase of both nucleotides (3.54 and 0.57 pmoles/mg DNA) when compared with the remaining endometrium (NIS) (2.70 and 0.30). The cAMP/cGMP ratio did not change significantly. We also found an increase in the proteins/mg DNA ratio (12.04 in IS vs 10.63 in NIS) and in the hydration of IS. Our hypothesis is that together these two nucleotides function as a monodirectional system in which they act cooperatively as positive effectors of different sequential steps in the overall process of implantation.
Assuntos
AMP Cíclico/análise , GMP Cíclico/análise , Implantação do Embrião , Endométrio/análise , Animais , Diferenciação Celular , Endométrio/citologia , Feminino , Gravidez , RatosRESUMO
The effects of chronic administration of three different doses of iprindole on the monoamine oxidase activity and neurotransmitter content were studied in the cerebral cortex, the mesencephalon and the cerebellum of mouse brain. The treatment inhibited mitochondrial monoamine oxidase activity of all three brain regions studied, although a dose-response inhibitory effect was found only in the mesencephalon. Brain regional serotonin contents were markedly increased after iprindole treatment. The 5-hydroxyindoleacetic acid contents showed a significant decrease only in the cerebral cortex. Neither dopamine nor norepinephrine brain regional contents were significantly altered. On the basis of these results it is suggested that a substrate-specific inhibition of monoamine oxidase is involved in the mechanism of action of this tricyclic antidepressant.
Assuntos
Encéfalo/efeitos dos fármacos , Indóis/farmacologia , Iprindol/farmacologia , Monoaminoxidase/metabolismo , Animais , Encéfalo/enzimologia , Cerebelo/efeitos dos fármacos , Córtex Cerebral/efeitos dos fármacos , Dopamina/metabolismo , Ácido Hidroxi-Indolacético/metabolismo , Masculino , Mesencéfalo/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/enzimologia , Muridae , Norepinefrina/metabolismo , Serotonina/metabolismoRESUMO
The presence of rabbit spermatozoa in the uterine horn of the doe over a 10-hr period induced an increase in the incorporation of leucine-14C and orotate-3H [from 230 +/- 50 to 520 +/- 32 and 146 +/- 41 to 432 +/- 40 (dpm 10(3) mg DNA; Mean +/- SEM)] into endometrial proteins and nucleic acids. The addition of actinomycin D or cyclohexamide significantly inhibited this process. The intraluminal administration of dibutyryl cyclic AMP (dbcAMP) (control group) induced an increase two times greater than that produced by spermatozoa. The increase in the incorporation of radioactive precursors into endometrial macromolecules induced by the spermatozoa is independent of the uterine carbonic anhydrase activation produced by this cell.
